| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | BAIG, Misbah | Hoffman Estates | 5:23.96 | |
| 2 | REZA, Alexandria | Belvidere (North) | 5:35.00 | |
| 3 | WILSON, Amanda | Minooka | 5:38.88 | |
| 4 | GALLOWAY, Elizabeth | Mt. Prospect (Prospect) | 5:41.56 | |
| 5 | SUAREZ, Yesenia | Joliet (West) | 5:55.42 | |
| 6 | SAMP, Emily | Lombard (Montini) | 5:56.54 | |
| 7 | MONK, Calissa | Buffalo Grove | 5:58.44 | |
| 8 | LOPEZ, Aileen | Morton | 5:58.81 | |
| 9 | STANISLAWSKI, Annie | Conant | 6:01.93 | |
| 10 | FORTMAN, Emma | Riverside (R.-Brookfield) | 6:13.50 | |
| 11 | LARSON, Grace | Roselle (Lake Park) | 6:17.15 | |
| 12 | CAREY, Alyssa | Bartlett | 6:27.00 | |
| 13 | VEGA, Mariah | Rockford (Guilford) | 6:30.00 | |
| 14 | TIERNEY, Shannon | Chicago (Taft) | 6:31.92 | |
| 15 | RICO, Kim | Belvidere | 6:33.30 |