| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | Luban, Rachel | SO | Rochelle Zell Jewish High School | 46.00 | Unverified: |
| 3 | Chambers, Quin' Niah | SO | Providence-St. Mel High School | 41.00 | Unverified: Estimated Time |
| 4 | Pabello, Maritoni | JR | CICS/Northtown High School | 38.26 | Verified |
| 5 | Mikolajewski, Alondra | SO | Cristo Rey Jesuit High School | 39.18 | Verified |
| 6 | Cope, Lily | FR | Rochelle Zell Jewish High School | 45.00 | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Thomas, Teanna | SO | CICS/Northtown High School | 36.32 | Verified |
| 2 | Wenzel, Alex | SO | Walther Christian Academy | 34.76 | Verified |
| 3 | Holman, Salene | FR | Walther Christian Academy | 30.54 | Verified |
| 4 | Taylor, Tyrah | SR | Hope Academy | 25.20 | Unverified: |
| 5 | Phillips, Aniya | JR | Hope Academy | 28.44 | Verified |
| 6 | Santana-bahena, Melisa | SR | Cristo Rey Jesuit High School | 34.60 | Verified |
| 7 | Jackson, Amira | FR | Christ the King Chicago | 35.07 | Verified |
| 8 | Anderson, Danada | FR | Providence-St. Mel High School | 38.00 | Unverified: Estimated Time |