| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 3 | CAMPELL, Eryon | Chicago (Providence-St. Mel) | NT |
| 4 | GARCIA-DYS, Brianna | Chicago (Cristo Rey Jesuit) | 1:23.70 |
| 5 | NELSON, Ariana | Chicago (Holy Trinity) | NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 2 | VERDUZCO, Carla | Melrose Park (Walther Christian) | 1:14.78 |
| 3 | CASTRO, Michelle | Melrose Park (Walther Christian) | 1:09.89 |
| 4 | TURNER, Moshay | Chicago (Holy Trinity) | 1:03.16 |
| 5 | ALLEN, Caitlyn | Chicago (Christ the King) | 1:06.37 |
| 6 | HARVEY, Chatera | Chicago (Christ the King) | 1:11.88 |
| 7 | SANTANA-BAHENA, Melisa | Chicago (Cristo Rey Jesuit) | 1:22.44 |