| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | WILLIS, Kiyun | Chicago (C. Hope Academy) | 50.59 |
| 2 | KROGER, Edward | Chicago (Christ the King) | 49.76 |
| 3 | DANG, Quang | Melrose Park (Walther Christian) | 48.35 |
| 4 | WILSON, Macrae | Chicago (C. Hope Academy) | 45.71 |
| 5 | MAGILL, Jonah | Rochelle Zell Jewish | 46.00 |
| 6 | GILDERSLEEVE, Jerrette | Melrose Park (Walther Christian) | 49.44 |
| 7 | KEYS, Jaylin | Chicago (Christ the King) | 49.94 |
| 8 | LAZARO, Javier | Chicago (Cristo Rey Jesuit) | 54.91 |