| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Bogan | A | NT | |
| 2 | Chicago (Crane) | A | DQ | |
| 3 | Chicago (Hancock) | A | NT | |
| 4 | Chicago (Simeon) | A | NT | |
| 5 | Chicago (Amundsen) | A | NT |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Chicago (Fenger) | A | NT | |
| 2 | Chicago (Von Steuben) | A | 5:10.00 | |
| 3 | The Noble Academy | A | NT | |
| 4 | ITW David Speer Academy | A | 4:44.48 | |
| 5 | Hansberry College Prep | A | 4:20.00 | |
| 6 | Chicago (Juarez) | A | DQ |