| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | Chicago (Prosser) | A | NT | |
| 4 | Chicago (Amundsen) | A | NT | |
| 5 | Chicago (Math & Science Charter) | A | NT |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Chicago (Kelvyn Park) | A | NT | |
| 2 | Chicago (Schurz) | A | NT | |
| 3 | Chicago (Lake View) | A | 1:15.00 | |
| 4 | ITW David Speer Academy | A | 55.72 | |
| 5 | Chicago (Taft) | A | 56.50 | |
| 6 | Chicago (Roosevelt) | A | NT | |
| 7 | Chicago (Rickover Naval Academy) | A | NT | |
| 8 | Chicago (Ogden International) | A | NT |