| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 4 | Culpepper, Egypt | FR | Chicago (Ogden International) | NT | |
| 5 | Nieves, Kayla | SR | Chicago (Kelvyn Park) | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Pernell, Ariel | FR | Chicago (Ogden International) | NT | |
| 2 | Chiong, Francine | SO | Chicago (Roosevelt) | NT | |
| 3 | Myles, Denise | FR | Chicago (Lake View) | 35.74 | |
| 4 | Ramos, Kassandra | FR | Chicago (Roosevelt) | 34.54 | |
| 5 | Zepeda, Maritza | SO | Chicago (Schurz) | 34.64 | |
| 6 | Hernandez, Frances | FR | Chicago (Rickover Naval Academy) | 39.04 | |
| 7 | Strom, Nicole | JR | Chicago (Rickover Naval Academy) | NT | |
| 8 | Rush, Tateanna | SR | Chicago (Kelvyn Park) | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Estrada, Jennifer | SR | Chicago (Von Steuben) | 33.60 | |
| 2 | Blease, Crystal | JR | Chicago (Uplift) | 32.00 | |
| 3 | Coleman, Armani | SO | Chicago (Senn) | 30.83 | |
| 4 | Marotta, Raena | FR | Chicago (Amundsen) | 30.57 | |
| 5 | Brown, Johntae | SR | Chicago (Schurz) | 30.77 | |
| 6 | Butzen, Joan | JR | Chicago (Taft) | 31.31 | |
| 7 | Williams, Jaylyn | JR | Chicago (Uplift) | 32.21 | |
| 8 | Espinoza, Sophia | SR | Chicago (Lake View) | 34.00 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Olea, Citlali | JR | ITW David Speer Academy | 30.14 | |
| 2 | Holcomb, Katie | SR | Chicago (Von Steuben) | 29.34 | |
| 3 | Courtney, Chartise | JR | Chicago (Prosser) | 27.97 | |
| 4 | Goodwin, Natasha | SR | Chicago (Prosser) | 27.13 | |
| 5 | Jackson, Nya | JR | ITW David Speer Academy | 27.17 | |
| 6 | Wells, Tylah | SO | Chicago (Senn) | 28.51 | |
| 7 | Bell, Ahniya | JR | Chicago (Amundsen) | 30.05 | |
| 8 | Przywara, Angela | SR | Chicago (Taft) | 30.20 |