| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Block, Dylan | SO | Harper College | 52.93 | NCC F.L. Slick Track & Field Invitational-2020 |
| 2 | Prohl, Christian | SO | Concordia (Wis.) |
52.67 | 2021 Warrior Invitational |
| 3 | DeBolt, Will | FR | Lewis |
51.89 | |
| 4 | Davis, Isaiah | FR | Lewis |
51.55 | |
| 5 | Jones, Jacoby | SO | Lewis |
49.96 | LRU Home Opener |
| 6 | Ragoo, Rackesh | FR | Lewis |
50.58 | Missouri S&T Miner Invitational |
| 7 | Spranger, Samual | SR | Concordia (Wis.) |
51.80 | |
| 8 | Brown, Jibreel | SR | Lewis |
51.90 | |
| 9 | Malek, Andrew | Unattached | 52.70 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | Simmons, Bradley | FR | Harper College | NT | |
| 4 | Horst, Joshua | SO | Concordia (Wis.) |
1:02.28 | Carroll Alumni Invitational |
| 5 | Hodge, Devery | FR | Benedictine (Ill.) |
53.22 | |
| 6 | Worley, Austin | SR | Illinois Tech |
58.70 | |
| 7 | Skinner, Xavier | SO | Triton | NT | |
| 8 | Garcia, John | FR | Harper College | NT |