| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Illinois Tech |
A | Krevitz | Moton | Muldrow | O'Donnell | 4:47.83 | 2020 NACC Indoor Championships |
| 4 | Lewis |
B | Pacer | Peinado | Renkes | Sanchez | 4:20.00 | |
| 5 | Concordia Univ. Chicago |
A | Nichols | Walters | Wingood | Almazan | DNF | |
| 6 | Lewis |
A | Amill-Ramos | Kozak | Learn | Kozak | 4:20.00 | |
| 7 | Aurora |
A | Etheridge | Gonzalez | Sorgenfrey | Liesen | 4:38.45 | |
| 8 | Aurora |
B | Rivera | Samp | Santiago | Robledo | 4:58.21 |