| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Minter, Nijae | FR | Knox | NT | |
| 2 | Heath, Elsa | JR | Principia | 1:14.73 | Principia Invite |
| 3 | Ware, Akilah | Millikin | 1:03.69 | ||
| 4 | Mitchell, Lydia | FR | Knox | 1:02.71 | Knox College Open |
| 5 | Johnson, Hollie | FR | Millikin | 1:03.66 | Houston Spring Break Invitational |
| 6 | Board, Aurora | FR | Lincoln College | 1:11.00 | Eastern Illinois Friday Night Special |
| 7 | Corzine, Rachel | FR | Knox | 1:21.20 | Monmouth Fighting Scots Indoor |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Kalisek, Kurstin | Northwestern University | 1:02.37 | ONU Invite 2019 | |
| 2 | Wimberly, Hailey | Millikin | 1:01.94 | ||
| 3 | Niebrugge, Brianna | SO | Millikin | 1:00.20 | CCIW Outdoor Track & Field Championships |
| 4 | Lukens, Erin | SR | Millikin | 56.75 | Greenville Select |
| 5 | Yoggerst, Lexi | JR | Illinois-Springfield | 58.07 | GLVC Outdoor Track & Field Championships |
| 6 | Renison, Eryn | FR | Millikin | 1:01.50 | |
| 7 | Moore, Malorie | U of I Club | 1:02.00 | ||
| 8 | Rance, Asia | SO | Lincoln College | 1:02.44 | Viking Olympics |