| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | DURU, Valencia | Full Stride | NT |
| 2 | MUCK, Mena | Revolution Express Track | 42.78 |
| 3 | SHAIFER-RIOS, Kali | Hilltop Speed | 41.65 |
| 4 | COTTON, Amina | Hilltop Speed | 37.51 |
| 5 | MANUAL, Tehya | Hilltop Speed | 39.06 |
| 6 | JOHNSON, Gianna | 38-Unattached | 41.78 |
| 7 | HANNEN, Carmen | B Sharp Academy | 1:13.89 |
| 8 | GAADI, Hannah | Sacramento Speed Factory | NT |