| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | ORTEGA, Elizabeth | Solano Elite Track Club | 1:07.00 |
| 2 | DAVIS, Jaliyah | Sacramento Speed Factory | 1:04.44 |
| 3 | PARKS, Dylan | Solano Elite Track Club | NT |
| 4 | KIELE, Madison | 38-Unattached | 56.30 |
| 5 | MUNOZ, Casey | Sacramento Speed Factory | 1:01.40 |
| 6 | ANDERSON, Marlee | Revolution Express Track | 1:06.82 |
| 7 | HENDERSON, Elise | Revolution Express Track | 1:16.69 |