| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | GUERRERO, Valentina | Revolution Express Track | 39.04 |
| 2 | MCKAY, Amaya | Revolution Express Track | 36.04 |
| 3 | OKOTIE, Violet | Full Stride | 34.36 |
| 4 | TAYLOR, Rubi | B Sharp Academy | 31.43 |
| 5 | ROBINSON, Miyah | Hilltop Speed | 33.45 |
| 6 | HARRINGTON, Olivia | Revolution Express Track | 35.05 |
| 7 | COBBS, Riley | Full Stride | 37.52 |
| 8 | COBBS, Peyton | Full Stride | 40.89 |