| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | RANDOLPH, Nina | Revolution Express Track | NT |
| 2 | MATHEWS-JOHNSON, Jemiah | Full Stride | 48.39 |
| 3 | GODTFREDSEN, Rayah | Revolution Express Track | 42.01 |
| 4 | GEKA, Torika | Revolution Express Track | 37.09 |
| 5 | HARRINGTON, Adelyn | Revolution Express Track | 34.13 |
| 6 | RICHARDSON, Kiera | Full Stride | 47.61 |
| 7 | CLARK, Mi'Amor | Hilltop Speed | NT |
| 8 | GAADI, Hannah | Sacramento Speed Factory | NT |