| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 1 | 69 | BASKERVILLE, Gianna | Fifty East Elite | NT |
| 2 | 158 | LAMONTAGNE, Autumn | Revolution Express Track | NT |
| 3 | 149 | ALLISON-KROG, Kaelyn | Revolution Express Track | 1:05.39 |
| 4 | 55 | PERRY, Jewel | FastFast Track Club | 57.57 |
| 5 | 234 | HUNTER, Honesty | Spikes and Speed | 1:00.45 |
| 6 | 42 | CARRICK, Josie | FastFast Track Club | 1:23.86 |
| 7 | 51 | NAIR, Ryan | FastFast Track Club | NT |
| 8 | 44 | DE SILVA, Sophia | FastFast Track Club | NT |