| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 1 | 49 | LYNCH, Charis | FastFast Track Club | NT |
| 2 | 113 | ANDERSON, Avianna | Rancho Cordova - RCTC | 46.55 |
| 3 | 118 | DUNCAN, Lauren | Rancho Cordova - RCTC | 42.44 |
| 4 | 201 | CHARLES, Callie | Sacramento Speed Factory | 36.77 |
| 5 | 92 | JONES, Hannah | Napa Valley Sprint | 37.84 |
| 6 | 198 | BOWERS, Ramiya | Sacramento Speed Factory | 45.68 |
| 7 | 115 | COOLBEAR, Quinn | Rancho Cordova - RCTC | NT |
| 8 | 50 | MURILLO, Arya | FastFast Track Club | NT |