| # | Athlete | Team | Seed | |
|---|---|---|---|---|
| 1 | 82 | BUXIE III, John | Legion of Zoom | 49.22 |
| 2 | 64 | LUND, Collin | Fifty East Elite | 49.96 |
| 3 | 224 | JACKSON, Jueli'en | Spikes and Speed | 50.57 |
| 4 | 63 | LOFTON, Trey | Fifty East Elite | 51.55 |
| 5 | 11 | BARNHART, Derek | FastFast Track Club | 51.98 |
| 6 | 242 | HANCOCK, Aiden | Unattached | 52.00 |
| 7 | 232 | THOMPSON, Jayden (JT) | Spikes and Speed | 52.81 |
| 8 | 8 | SAUNDERS, Brody | Ace Track Club | 53.21 |
| 9 | 66 | PETINAUD I, Christian | Fifty East Elite | 53.36 |
| 10 | 89 | RODRIGUEZ, Alfredo | Napa Valley Sprint | 54.06 |
| 11 | 182 | PARROW, Damien | Sacramento Speed Factory | 54.24 |
| 12 | 171 | CROCKER, Jayden | Sacramento Speed Factory | 54.54 |
| 13 | 36 | WHEATON, Riley | FastFast Track Club | 1:11.53 |
| 14 | 222 | COLLINS, Romel | Spikes and Speed | NT |
| 15 | 144 | MURPHY, Deshawn | Revolution Express Track | NT |
| 16 | 168 | WILLIAMS, Brayden | Ruby Jewell Track & Field Cl | NT |