| # | Athlete | Team | Seed | |
|---|---|---|---|---|
| 1 | 167 | WINSTON, Alysiana | Revolution Express Track | 24.25 |
| 2 | 159 | MARTIN, Aniya | Revolution Express Track | 25.34 |
| 3 | 55 | PERRY, Jewel | FastFast Track Club | 25.78 |
| 4 | 155 | FLYNN, Zayna | Revolution Express Track | 26.21 |
| 5 | 202 | COLLIER, Myah | Sacramento Speed Factory | 26.90 |
| 6 | 234 | HUNTER, Honesty | Spikes and Speed | 27.37 |
| 7 | 212 | STEWARD, Alexis | Sacramento Speed Factory | 27.57 |
| 8 | 44 | DE SILVA, Sophia | FastFast Track Club | 29.23 |
| 9 | 199 | BUFORD, Kimari | Sacramento Speed Factory | 30.52 |
| 10 | 42 | CARRICK, Josie | FastFast Track Club | 33.60 |
| 11 | 214 | TURNER, Alivia | Sacramento Speed Factory | NT |
| 12 | 69 | BASKERVILLE, Gianna | Fifty East Elite | NT |