| # | Athlete | Team | Seed | |
|---|---|---|---|---|
| 1 | 216 | WILSON, Nariyah | Sacramento Speed Factory | 25.99 |
| 2 | 197 | BOSLEY, Londyn | Sacramento Speed Factory | 26.50 |
| 3 | 148 | ALEXANDER, Mia | Revolution Express Track | 26.88 |
| 4 | 94 | JONES, Keira | Napa Valley Sprint | 27.07 |
| 5 | 235 | JORGENSEN, Harleigh | Spikes and Speed | 28.30 |
| 6 | 236 | LARA, Ava | Spikes and Speed | 28.96 |
| 7 | 43 | CHISSIE, Taylor | FastFast Track Club | 29.50 |
| 8 | 90 | CERLETTI, Samantha | Napa Valley Sprint | 29.85 |
| 9 | 91 | DUDEN, Ava | Napa Valley Sprint | 30.81 |
| 10 | 207 | MCBRIDE, Aaliyah | Sacramento Speed Factory | 30.83 |
| 11 | 54 | PENA, Nevaeh | FastFast Track Club | 30.95 |
| 12 | 210 | POYER, Starr-Kailee | Sacramento Speed Factory | 31.22 |
| 13 | 160 | NOLAN, Melayah | Revolution Express Track | 31.98 |
| 14 | 156 | GANDHOKE, Jasleen | Revolution Express Track | NT |
| 15 | 45 | HEISE, Nia | FastFast Track Club | NT |
| 16 | 237 | SOUTH, Kaylah | Spikes and Speed | NT |
| 17 | 40 | BROWN, Rylee | FastFast Track Club | NT |
| 18 | 85 | ZIMMERMAN, Madeline | Legion of Zoom | NT |