| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Baldwin |
A | Scippio | Pitts | Decker | Thomas | 4:59.15 | Andrew Jackson Tiger Invitational |
| 3 | Terry Parker |
A | Abbott | Abbas | Jones | Russell | 4:30.65 | FHSAA 3A Region 1 |
| 4 | Riverside |
A | Coley | Williams | Shuford | Blackshear | 4:22.41 | Bob Hayes High School Invitational |
| 5 | Atlantic Coast |
A | Evans | Martinez | Gilchrist | Anthony | 4:25.94 | FHSAA 4A District 2 |
| 6 | Ed White |
A | Kirby | Pryce | Rodriguez | George | 4:38.59 | Baldwin Home Meet #2 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Ribault |
A | Webber | Sykes | Hagans | Gardner | 4:16.76 | Gateway Conference Track & Field Championship |
| 3 | Raines |
A | Alston | Jones | Middleton | Smothers | 4:09.56 | FHSAA 2A District 4 |
| 4 | Andrew Jackson |
A | Brown | Bartley | Barrington | Kelly | 3:55.26 | Bob Hayes High School Invitational |
| 5 | First Coast |
A | Dudley | McWhite | Marseille | King | 4:06.28 | FHSAA 4A District 2 |
| 6 | Westside |
A | Chretien | Fanning | Marshall | Taylor | 4:12.82 | 2024 Lewis James Invitational |