| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Eagles View |
A | Amos | Higdon | Singleton | Willard | NT | |
| 2 | Riverside |
A | Wiggins | Williams | Henry | Billings | 43.16 | FHSAA 3A Region 1 |
| 3 | Paxon |
A | Gardner | Okor | Davis | Richard | 42.54 | FHSAA 2A Region 1 |
| 4 | Raines |
A | Ford | Kaye | Downer-Barrett | Kilpatrick | 42.17 | Florida Relays |
| 5 | Atlantic Coast |
A | Bell | Love | Evans | Orsborn II | 42.05 | FHSAA 4A District 2 |
| 6 | Ribault |
A | Francis | Shaw | McDuffie | Harrington | 42.35 | FHSAA 2A District 4 |
| 7 | Sandalwood |
A | Anderson | Blount | Foster | Williams | 42.55 | TFRRS 04/26/22 - 43 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ed White |
A | Thomas | Mercer | Perry | Hampton | 42.72 | FHSAA 2A District 3 |
| 2 | Fernandina Beach |
A | Billings | Kallstrand | Boyd | Poe | 44.91 | FHSAA 2A District 4 |
| 3 | Terry Parker |
A | Grundy | Ragin | Taiwo | Jackson | 43.68 | Adrain Mann Wolfpack Classic (40th Annual) |
| 4 | Episcopal |
A | Buchanon | Kaufman | Kemp | Williams | 43.75 | FHSAA 2A Region 1 |
| 5 | First Coast |
A | Harriel | Bowen | Colbert | Evans | 43.31 | Adrain Mann Wolfpack Classic (40th Annual) |
| 6 | Wolfson |
A | Carter | Grooms | Blake | Richards | 46.36 | Adrain Mann Wolfpack Classic (39th Annual) |
| 7 | Westside |
A | Randall | Taylor | Green | Lewis | 41.99 | FHSAA 3A Region 1 |