| # | Stadium Record | 48.32 | Lakewood HS | Lakewood HS | 2/20/2016 |
| # | Stadium Record | 48.32 | Chadwick, Garcia, Jenkins, St. Fleur, Walker, White | Lake Wales HS | 3/4/2023 |
| $ | Meet Record | 48.32 | Chadwick, Garcia, Jenkins, St. Fleur, Walker, White | Lake Wales | 3/4/2023 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Note |
|---|---|---|---|---|---|---|---|
| 1 | Haines City |
A | Canaday | Thomas | Holmes | Hurst | |
| 2 | Berkeley Prep |
A | Aves | Hankerson | Lopez del Valle | Raffington | |
| 3 | Winter Haven |
A | Adonteng | Conoly | Kerlegrand | Murray | Newsome Wolves Invitational 2024 |
| 4 | Lake Wales |
A | Alston | St. Fleur | Mickel | Walker | FHSAA 3A Region 2 |
| 5 | George Jenkins |
A | Gibson | Albritton | Rose | Gallishaw | FHSAA 4A Region 2 |
| 6 | Tampa Catholic |
A | Williams | D'Almeida | Bangos | Loyd | FHSAA 2A Region 3 |
| 7 | Lakeland |
A | Davis | Canales | Dekle | Matthews | |
| 8 | Mitchell |
A | Tomlin | Thuma | Hillmon | Unger | Anclote Sharks Early Bait 2024 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Note |
|---|---|---|---|---|---|---|---|
| 3 | George Jenkins |
C | Jenkins | Smith | Williams | Gibson | |
| 4 | Clearwater |
A | Agalla | Agyekum | Daniels | Devine | FHSAA 3A District 10 |
| 5 | George Jenkins |
B | Williams | Black | Bradley | Arrington |