| # | Stadium Record | 41.22 | Dunbar HS | Dunbar HS | 4/27/2017 |
| $ | Meet Record | 41.39 | Abrams, Fletcher, Whitfield, Singleton | Dunbar HS | 3/4/2023 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Note |
|---|---|---|---|---|---|---|---|
| 1 | Bishop Verot |
A | Young | Young | Turner | Gore | 2021 Private 8 Track Meet |
| 2 | Tampa Catholic |
A | Dimaria | Phillips | Mitchell | Douglas | FHSAA 2A Region 3 |
| 3 | Clearwater |
A | Berrien | White | Loving | Simes | FHSAA 3A Region 3 |
| 4 | Lake Wales |
A | Orr | Daniels | Filsaime | Alls | |
| 5 | Wesley Chapel |
A | Montalvo-Luna | Chisanga Jr | Johnson | Falcon | FHSAA 3A Region 2 |
| 6 | Winter Haven |
A | Ervin | Williams | Callaway | Tarver | |
| 7 | Lakeland |
A | Dobie | Dobie | Jackson | BATTLE | |
| 8 | Berkeley Prep |
A | Cooper | Dost | Wehrell | Green |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Note |
|---|---|---|---|---|---|---|---|
| 2 | Berkeley Prep |
B | Aurelien | King | Gills | George | |
| 3 | Carrollwood |
A | Song | Robinson | Earle | Johnson | |
| 4 | George Jenkins |
A | Mapp | Harris | Capron | Murray | |
| 5 | Haines City |
A | Bordes | Augustin | James | Deyant | |
| 6 | Bishop Verot |
B | Clark | Thompson | Gonzales | Turner | 2021 Private 8 Track Meet |
| 7 | Auburndale |
A | Blocker | McRoy | Mcgough | Jackson |