Qualifier Winners gets Preferred Lanes (4-5-3-6)
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CROCKETT, Morgan | SO | St. Thomas Aquinas | 25.44 | q |
| 2 | ISAAC, Kynah | SR | Flanagan | 24.84 | q |
| 3 | SHAW, Ashley | SO | Northeast (OP) | 25.65 | Q |
| 4 | CARTER, Jassani | JR | Flanagan | 24.83 | Q |
| 5 | FACEY, Christin | FR | Nova | 25.31 | Q |
| 6 | MCCALLA, Annyah | JR | Ely | 26.13 | Q |
| 7 | BLACKWOOD, Jody | JR | Miramar | 25.44 | q |
| 8 | MUNDELL, Melissa | SR | Nova | 25.61 | q |