Qualifier Winners gets Preferred Lanes (4-5-3-6)
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MCCLOSKEY, Haley | SR | St. Thomas Aquinas | 5:34.29 | q |
| 2 | ROBERTS, Kashi | SR | Dillard | 5:31.71 | q |
| 3 | POTENZANO, Gianna | SO | Monarch | 5:43.93 | Q |
| 4 | DUFFNER, Lindsey | FR | St. Thomas Aquinas | 5:19.36 | Q |
| 5 | COLEMAN, Christiana | FR | Dillard | 5:31.02 | Q |
| 6 | ALICEA, Daniela | SR | West Broward | 5:51.79 | Q |
| 7 | MARTINEZ, Danielle | SR | St. Thomas Aquinas | 5:33.15 | q |
| 8 | KAUFMAN, Anika | SO | Cypress Bay | 5:34.92 | q |