| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Note |
|---|---|---|---|---|---|---|---|
| 3 | Mandarin |
A | Jackson | Hodge | Jolly | Williams | FHSAA 4A Region 1 |
| 4 | Ribault |
A | Oakley | Rolle | Sharpe | Shaw | 2023 Chandra Cheeseborough Invitational - High School |
| 5 | Ribault |
B | Clarke | Jerkins | Bush | Stevens | 2023 Chandra Cheeseborough Invitational - High School |