| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Note |
|---|---|---|---|---|---|---|---|
| 1 | Highland Christian | A | Milligan | Mclane | Wartman | Bozeman | 5:54.06 Mar 28%2C 2026%3B Harmon%27s Invitational |
| 2 | Lafayette |
A | De Reu | Posada | Sparrow | Pilkington | Branford 2026 |
| 3 | Suwannee |
A | Lewis | Siemon | Ouedraogo | Bricker | FSU Relays (High Schools) 2026 |
| 4 | Hamilton County |
A | Wallace | Johnson | Aldama-Vaquero | Robinson | |
| 5 | Columbia |
A | Todd | Thompson | Todd | Thompson | Chiefland Home Meet 3 |
| 6 | Trenton |
A | Diez | Jones | Dewitz | Wilson | Chiefland Home Meet 3 |