| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Note |
|---|---|---|---|---|---|---|---|
| 2 | Hamilton County |
A | Johnson | Rayford | Marshall | Berry | Columbia High Tiger Invitational |
| 3 | Columbia |
A | Newton | Moore | Cooper | Caldwell | Columbia High Tiger Invitational |
| 4 | Lafayette |
A | Anderson | Howard | Plunkett | Stewart | Branford 2026 |
| 5 | Suwannee |
A | Haley | Stoltzfoos | Skinner | Daniels | Tariq Barfield Invitational (Approved Qualifier) |