8 Fastest Times Will Be In 2nd Heat; Qualifier Winners Will Get Preferred Lanes (4-5-3-6)
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Damari SIBBLES | JR | Taravella |
1:01.89 | |
| 2 | Trenayah MILES | JR | Flanagan |
1:01.83 | |
| 3 | Kaniyah JOHNSON | SR | Dillard |
1:01.34 | |
| 4 | Shanel MORGAN | JR | St. Thomas Aquinas |
1:00.79 | South Central Winner |
| 5 | Katrice BURKE | JR | Monarch |
1:00.74 | North Runner-Up |
| 6 | Tatiana SPRADLEY | SR | Flanagan |
1:01.57 | |
| 7 | Alexcia LIVERPOOL | SO | Chaminade-Madonna |
1:01.88 | South Central Runner-Up |
| 8 | Hannah COMPTON | JR | Cypress Bay |
1:01.91 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Alani WILLIAMS | SO | Fort Lauderdale |
59.89 | |
| 2 | Abigail MULLINGS | SR | Northeast (OP) |
58.78 | |
| 3 | Amelia CHRISTOPHER | FR | Coral Springs |
1:00.19 | North Winner |
| 4 | Shynah COLLINS | 7 | Dillard |
55.14 | North Central Winner |
| 5 | Lenee BARBARY | FR | Flanagan |
59.00 | South Winner |
| 6 | Ajah ARSCOTTT | SR | Taravella |
56.93 | North Central Runner-Up |
| 7 | Zareya BRADWELL | SR | Dillard |
59.01 | |
| 8 | Briauna MORENCY | JR | Flanagan |
1:00.42 | South Runner-Up |