8 Fastest Times Will Be In 2nd Heat; Qualifier Winners Will Get Preferred Lanes (4-5-3-6)
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Pembroke Pines Charter |
A | Monagas | Benavides | Jean Louis | Rogers | 4:40.89 | |
| 2 | Monarch |
A | Burke | Whyte | Stephenson | Hannah | 4:37.15 | |
| 3 | Blanche Ely |
A | Dennis | Lyons | Hendricks | Gore | 4:28.01 | |
| 4 | Cypress Bay |
A | Compton | Dubuisson | McKinney | Miletti | DNS | South Central Winner |
| 5 | Miramar |
A | Howard | Mccrackan | Modile | Manning | 4:27.35 | |
| 6 | Cardinal Gibbons |
A | Mayo | Albrecht | Hickox | Charles | DNS | |
| 7 | Nova |
A | Griffin | Barrett | Paul | Foster | 4:40.28 | South Central Runner-Up |
| 8 | Stranahan |
A | Morgan | Naves | Collier | Garcia | 4:45.88 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Pompano Beach |
A | Franco | Christie | Essiet | Richardson | 4:08.87 | North Runner-Up |
| 2 | Northeast (OP) |
A | Compere | Mullings | Jean | Parker | 4:00.26 | |
| 3 | Flanagan |
A | Spradley | Morency | Miles | Schubert | 4:10.66 | South Winner |
| 4 | Taravella |
A | Sibbles | Spann-Pierre | Taylor | Arscottt | 3:57.49 | North Central Winner |
| 5 | Coral Springs |
A | Alvaranga | Christopher | Higgins | Gentilhomme | 4:06.98 | North Winner |
| 6 | Dillard |
A | Reid | Webb | Edwards | Collins | 3:58.55 | North Central Runner-Up |
| 7 | Boyd Anderson |
A | Jean | Mcdonald | Taylor | Frazier | 4:06.23 | |
| 8 | South Broward |
A | Tanis | Spence | Torres-Martinez | Ricks | 4:24.67 | South Runner-Up |