8 Fastest Times Will Be In 2nd Heat; Qualifier Winners Will Get Preferred Lanes (4-5-3-6)
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | McArthur |
A | Rutherford | Ramirez | Johnson | Marcellus | 3:35.57 | |
| 2 | Stranahan |
A | Armstrong | Butler | Souffrant | Delva | 3:34.24 | |
| 3 | Piper |
A | Trinidad | Conoway | Wright | Edouard | 3:30.88 | |
| 4 | Coral Glades |
A | Dennis | Burrell | Tomlin | Blaise | DNS | North Winner |
| 5 | Monarch |
A | Harris | Savio | Potenzano | Robinson | 3:30.79 | North Runner-Up |
| 6 | Pembroke Pines Charter |
A | AKINBEHINJE | Oladapo | Schwartz | Fenn | 3:34.21 | |
| 7 | Blanche Ely |
A | Harrison | Smith | Hines | Goldsby | 3:35.23 | |
| 8 | Plantation |
A | Alexander | Braddy | Burnett | Thomas | 3:35.98 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St. Thomas Aquinas |
A | Chamberlain | Mitchell | Horne Jr. | Adams III | 3:27.04 | South Central Runner-Up |
| 2 | Northeast (OP) |
A | Campbell | Brown | Baptiste | Parke | 3:24.57 | |
| 3 | Western |
A | Tkachenko | Meneses | Ruskin | Suarez-Davis | 3:25.26 | South Central Winner |
| 4 | Boyd Anderson |
A | Watson | Stewart-McLaren | Jeoboham | Bethely | 3:19.64 | North Central Winner |
| 5 | Miramar |
A | Baptiste | Boucard | Reid | Smith | 3:25.22 | South Winner |
| 6 | Dillard |
A | Jennings | Kellem | Joseph | Sapp | 3:20.85 | North Central Runner-Up |
| 7 | Fort Lauderdale |
A | King | Losse | Manca | Peeples | DNS | |
| 8 | Flanagan |
A | Johnson | Baldie | Evans | Carter | 3:27.26 | South Runner-Up |