8 Fastest Times Will Be In 2nd Heat; Qualifier Winners Will Get Preferred Lanes (4-5-3-6)
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | West Broward |
A | Williams | Leon Suarez | Williams | Walker | 53.35 | |
| 2 | McArthur |
A | Lloyd | Johnson | Francois | Lopez | 52.58 | South Runner-Up |
| 3 | Monarch |
A | Johnson | Burke | Whyte | Stephenson | 51.27 | |
| 4 | Coral Glades |
A | Hermitt | Camper | Swain | Martinez | 50.95 | |
| 5 | Blanche Ely |
A | McFadden | Goldwire | kelly | Rogers | 50.95 | |
| 6 | Taravella |
A | Tenn | Marriott | Alvarez | Taylor | 52.25 | |
| 7 | Hollywood Hills |
A | Santana | Mortimer | Hackett | SCOTT | DNS | South Central Runner-Up |
| 8 | Everglades |
A | menard | parkinson | wint | baez | 54.15 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Plantation |
A | Fortulein | Evans | Dawes | Fevoy | 49.94 | |
| 2 | Boyd Anderson |
A | Frazier | Taylor | Mcdonald | Jean | 48.86 | North Central Runner-Up |
| 3 | Flanagan |
A | Jones | Okereke | Joyce | Morgan | 49.70 | South Winner |
| 4 | Dillard |
A | Christian | Cobb | Webb | baker | 47.84 | North Central Winner |
| 5 | Pompano Beach |
A | Christie | Essiet | Richardson | Colon | 48.74 | North Winner |
| 6 | Nova |
A | Fulks | Souffrant | Cothiere | Devoe | 50.79 | South Central Winner |
| 7 | Douglas |
A | Faust | Freckleton | Osouna | Grissette | 49.88 | North Runner-Up |
| 8 | Coral Springs |
A | Alvaranga | Anestal | Samuels | Poitier | 50.06 |