| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Fletcher | A | Holtz | Hankerson | Fox | Ali | DNS | Coach Mac & Brown Saints Open |
| 2 | Riverside | A | Pooler | Brown | McKenzie | Oruamabo | DNS | Mandarin Mustang Classic |
| 3 | Westside | A | Davis | McCrae | Drake-Jackson | Flomo | 3:50.07 | Bear Classic / True Team Championships |
| 4 | Ed White | A | Patterson | Patterson | Simms | Mays | 3:38.26 | Stanton Prep Track & Field Meet |
| 5 | Atlantic Coast | A | Sea | Cardenas | Davis | Carter | 3:42.80 | Bear Classic / True Team Championships |
| 6 | Wolfson | A | Reynolds | Melton | Maclean | Maxwell | 3:51.32 | Stanton Prep Track & Field Meet |
| 7 | Stanton | A | Agarwal | Patel | Arline-Hardman | Emmanuel | DNS | Stanton Prep Track & Field Meet |
| 8 | Baldwin | A | Brooks | Skidmore | DNS | Ed White Mini Track & Field Meet |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | First Coast | A | Davis | Morris-Heron | Harrison | Saunders | 3:35.47 | Ed White Mini Track & Field Meet |
| 2 | Terry Parker | A | Glover | Williams | Dorsey Jr | Sinclair | 3:29.55 | Stanton Prep Track & Field Meet |
| 3 | Sandalwood | A | Copes | Tankard | Terry | Ward | 3:27.48 | Stanton Prep Track & Field Meet |
| 4 | Raines | A | Burroughs | King | Collier | Kirkland | 3:17.49 | Florida Relays |
| 5 | Andrew Jackson | A | Bruist | Williams | Johnson | Folson | 3:26.03 | Mandarin Mustang Developmental Meet 1 |
| 6 | Mandarin | A | Summerall | Lewis | Brown | Degregorio | 3:29.05 | Don Hall Invitational |
| 7 | Ribault | A | Davis | Axon | Morales | Rodgers | 3:33.98 | Bob Hayes Classic |
| 8 | Paxon | A | Anderson | Broomfield | Terrell | Taylor | 3:35.92 | Stanton Prep Track & Field Meet |