| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | DIETRICH, Victoria | PSU-Behrend |
1:06.91 | SRU Open |
| 4 | LAFFAN, Ivy | PSU-Behrend |
1:00.20 | Tomcat Invitational |
| 5 | JACOBS, Lauren | Edinboro |
1:01.67 | Dave Labor Invitational |
| 6 | QUESENBERRY, Megan | Penn State T&F Club | 1:10.00 |