| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | BUHL, Rachael | PSU-Behrend |
32.50 | Steemer Showcase |
| 4 | JONES, Grace | PSU-Behrend |
31.18 | SRU Open |
| 5 | ANAYA, Abby | Wheaties Box Rejects | 38.00 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | CAMPBELL, Maria | PSU-Behrend |
30.32 | Erie County PA - North of 90 Meet |
| 2 | DIETRICH, Victoria | PSU-Behrend |
29.21 | Westminster Invitational 2022 |
| 3 | WILLIAMS, Amanda | Edinboro |
27.98 | |
| 4 | LAFFAN, Ivy | PSU-Behrend |
27.13 | Westminster Invitational 2022 |
| 5 | JENKINS, Andrea | Penn State T&F Club | 27.50 | |
| 6 | KAPPELER, Madison | PSU-Behrend |
28.90 | Ashland Jud Logan Light Giver Open Collegiate Indoor |
| 7 | GOURLEY, Megan | Edinboro |
30.08 | Westminster Invitational 2022 |
| 8 | SKOCZLAS, Rachel | PSU-Behrend |
30.57 | Westminster Invitational 2022 |