| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | John Brown |
A | 46.61 | JBU SAC Invitational |
| 3 | Southwestern Assemblies of God |
B | 43.00 | |
| 4 | Bethany (Kan.) |
A | 42.34 | |
| 5 | Bethel (Kan.) |
A | 42.46 | |
| 6 | Langston |
B | DNS |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Allen CC | A | 41.62 | |
| 2 | Friends |
A | DNS | |
| 3 | Southwestern Assemblies of God |
A | 41.24 | NAIA Championships |
| 4 | Langston |
A | 40.94 | Langston Invitation |
| 5 | Southwestern (Kan.) |
A | 41.20 | |
| 6 | Southwestern (Kan.) |
B | 41.40 | |
| 7 | Tabor |
A | 41.60 | 2022 Tabor College Invite |
| 8 | Cowley College | A | 42.00 |