| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Sterling | A | 52.18 | Moundridge Invitational 2019 |
| 2 | Cheney | A | 51.14 | Dodge City%2C March 31%2C 2022 |
| 3 | Wichita-Kapaun-Mt Carmel | A | 50.44 | Wichita Southeast 2022 |
| 4 | Derby | A | 49.72 | AVCTL Division 1 League Track Meet |
| 5 | Eudora | A | 50.20 | |
| 6 | Valley Center | A | 50.74 | AVCTL Division 2 League Track Meet |
| 7 | Andale | A | 51.30 | Paul Schmidt Invitational |
| 8 | Hutchinson | A | DNF |