| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Derby | A | 43.97 | |
| 2 | Bethany | A | 43.81 | |
| 3 | Rose Hill | A | 43.57 | |
| 4 | Valley Center | A | DQ | AVCTL Division 2 League Track Meet |
| 5 | Chanute | A | 43.54 | Labette County 3/31/22 |
| 6 | Eudora | A | 43.80 | |
| 7 | Wichita-Trinity | A | 43.93 | 2021 CPL League Track Meet |
| 8 | Wichita-Bishop Carroll | A | 44.11 | Andale 4/1/22 |