| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | PETERSON, LAURYN | NEOSHO COUNTY CC | 1:12.48 | 15th Annual David Suenram Gorilla Classic |
| 2 | SCHROEDER, PARKER | BETHEL (KAN.) |
1:04.27 | Friends University Spring Invitational |
| 3 | TERRY, LAURYN | SOUTHWESTERN (KAN.) |
1:01.63 | Wichita State KT Woodman Classic |
| 4 | HANNA, MELIAH | SOUTHWESTERN (KAN.) |
1:00.36 | Wichita State KT Woodman Classic |
| 5 | ROMAN, MIA | BETHEL (KAN.) |
1:01.00 | 2023 Coyote Distance Carnival |
| 6 | VAZQUEZ, ESTHER | COWLEY COLLEGE | 1:03.71 | Wichita State KT Woodman Classic |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | ENGERMANN, RENAJA | NORTHWESTERN OKLAHOMA |
59.64 | PR high school |
| 2 | MILLNER, FAITH | COFFEYVILLE CC | 58.86 | https://ok.milesplit.com/athletes/8646360-faith-millner |
| 3 | PEREZ, VANESSA | OKLAHOMA WESLEYAN |
58.00 | |
| 4 | KUPKA, ISABELLA | COWLEY COLLEGE | 57.02 | NJCAA Indoor Championships |
| 5 | ROBINSON, ASHLEY | ALLEN CC | 57.68 | John McDonnell Invitational |
| 6 | HILL, KYAH | SOUTHWESTERN (KAN.) |
58.07 | 2023 Tabor Invite |
| 7 | DABNEY, VARAIE | ALLEN CC | 59.45 | |
| 8 | LANE, SHAKIRA | NORTHWESTERN OKLAHOMA |
1:00.06 | Emporia State Relays |