| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 3 | PAXSON, Abigail | Argonia | 55.21 |
| 4 | ROBBEN, Isabella | Cheney | 59.70 |
| 5 | REESE, Alayna | Norwich | 1:03.50 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | ELLIS, Hannah | Cunningham | 55.14 |
| 2 | MORRISON, Tori | Argonia | 55.06 |
| 3 | SMITH, Reagan | Norwich | 54.38 |
| 4 | WIESE, Erika | Cheney | 53.01 |
| 5 | BRYANT, Logan | Goessel | 53.34 |
| 6 | GRIFFIN, Trinity | Cheney | 54.50 |