| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | BII, WINNY | OKLAHOMA STATE |
NT | |
| 3 | ANDERSON, MA'LIYAH | WICHITA STATE |
1:03.78 | Wichita State Herm Wilson Invitational |
| 4 | PETTY, KAYLAH | FRIENDS |
1:01.98 | Friends University Spring Open |
| 5 | WARREN, KOURTNEY | FRIENDS |
1:02.14 | Wichita State Shocker Spring Invitational |
| 6 | FACTOR, KAYCEE | KANSAS CITY |
1:05.10 | Jayhawk Classic |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | KNIGHT, JAYLEN | FRIENDS |
59.20 | |
| 3 | JOHNSON, SYDNEY | WICHITA STATE |
56.81 | KU-KSU-WSU Triangular |
| 4 | MAGER, ANNA | TULSA |
56.40 | |
| 5 | HACKNEY-COOPER, ELONTAE | UNAT-WICHITA STATE | 57.52 | Wichita State Shocker Open |
| 6 | HOLTERMANN, LIANNE | TULSA |
56.80 | |
| 7 | HANGER, MADDIE | OKLAHOMA STATE |
59.00 |