| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | MATHEWS, AMRIAH | KANSAS |
28.32 | Jayhawk Classic |
| 3 | IMAHORI, KOTO | IOWA CENTRAL CC |
27.37 | 2022 Region XI NJCAA Championships |
| 4 | TAYLOR, QUEANNA | IOWA CENTRAL CC |
26.65 | NJCAA Region 11 Championship |
| 5 | BII, WINNY | OKLAHOMA STATE |
NT | |
| 6 | FREY, ABIGAIL | IOWA CENTRAL CC |
27.08 | SDSU Indoor Classic |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | ANDERSON, TIARA | KANSAS |
26.15 | 2022 ORU Invite |
| 3 | HEACOCK, ANNA | UNAT-WICHITA STATE | 25.70 | The American Outdoor Track & Field Championships |
| 4 | HOLTERMANN, LIANNE | TULSA |
25.40 | |
| 5 | LANEAU, RANIYA | TULSA |
25.40 | |
| 6 | ANDERSON, MA'LIYAH | WICHITA STATE |
26.11 | Arkansas Qualifier |
| 7 | HANCOCK, RYLIE | TULSA |
26.25 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | WRIGHT, SATANYA | KANSAS |
25.02 | KSU Ward Haylett Invitational |
| 3 | HACKNEY-COOPER, ELONTAE | UNAT-WICHITA STATE | 25.17 | Grizzly Classic |
| 4 | ROCA, ELIZABETH | TULSA |
24.45 | |
| 5 | SIMMONS, SAVANNAH | WICHITA STATE |
24.74 | |
| 6 | GIBSON, JAYDA | TULSA |
24.55 | |
| 7 | MAGER, ANNA | TULSA |
24.50 |