| Athlete | Team | Seed | Note | |
|---|---|---|---|---|
| 1 | CARTER, KAMIJAH | IOWA CENTRAL CC |
51.00 | |
| 2 | PENNER, TOBY | WICHITA STATE |
52.23 | NAIA Outdoor Track & Field Championships |
| 3 | WILMINGTON, CAMERON | KANSAS |
52.45 | Rock Chalk Classic |
| 4 | COLBERT, JAMEIR | KANSAS |
53.60 | |
| 5 | BISHOP, TAFARI | IOWA CENTRAL CC |
54.70 | Wichita State Shocker Spring Invitational |
| 6 | WEHNER, CADEN | KANSAS CITY |
54.89 | |
| 7 | MONTGOMERY, JALEEL | WICHITA STATE |
NT | |
| 8 | HANEY, BRYAN | WICHITA STATE |
NT |