| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 2 | STARKS, Aariah | James Monroe | NT |
| 3 | STOWERS, Justise | Narrows | 1:05.23 |
| 4 | HUBBARD, Gracie | James Monroe | NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | WHITE, Annabelle | Giles | 58.25 |
| 2 | KIRBY, Jackie | Narrows | 57.12 |
| 3 | HOUCK, Gabriella | James Monroe | 56.86 |
| 4 | BREWSTER, Bailey | Princeton Senior | 57.04 |
| 5 | REED, Karsyn | Narrows | 57.22 |
| 6 | BAILEY, Brooklyn | Narrows | 1:00.21 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | WHITT, Hannah | Princeton Senior | 55.53 |
| 2 | HAWKINS, Leah | George Wythe (Wytheville) | 54.00 |
| 4 | LEON, Fiorella | George Wythe (Wytheville) | 51.26 |
| 5 | OZMAR, Bella | George Wythe (Wytheville) | 55.00 |
| 6 | GULIFOYLE, Malia | Giles | 56.62 |