| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | Rhyan Dollar-Daugherty | Joshua | 2:28.26 |
| 2 | Alexus Vasquez | Sanger | 2:33.15 |
| 3 | Citlali Lopez | Mineral Wells High School | 2:38.53 |
| 4 | Leilani Woods | Denton | 2:50.47 |
| 5 | Alli Hamond | Joshua | 2:51.27 |
| 6 | Amber Cook | Mineral Wells High School | 2:55.28 |
| 7 | McKenzie Rittener | Frisco Emerson | NT |
| 8 | Gracie Grahl | Sanger | NT |
| 9 | Taniea Jackson | Frisco Emerson | NT |
| 10 | Audrey Carver | Frisco Emerson | NT |