| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | Catie Rennick | Denton | 6:17.37 |
| 2 | Bailey Dewey | Sanger | 6:21.70 |
| 3 | Hailie Schmidt | Sanger | 6:29.19 |
| 4 | Jayden Wales | Frisco Emerson | 6:46.65 |
| 5 | Heidi Smith | Denton | 6:53.80 |
| 6 | Abril Diaz | Mesquite | 6:57.14 |
| 7 | Isabella Baier | Frisco Emerson | 7:04.49 |
| 8 | Yiritza Trejo | Mesquite | 7:15.67 |
| 9 | Emily Rangel | Denton | 7:27.00 |
| 10 | Arionna Gossett | West Mesquite | 8:16.10 |
| 11 | Kiddie Zamora | Frisco Emerson | NT |
| 12 | Erica Wright | West Mesquite | NT |
| 13 | Emma Ramos | West Mesquite | NT |
| 14 | Ximena Valerio Ortiz | Mesquite | NT |
| 15 | Braylee Butterfield | Sanger | NT |
| 16 | Katerine Bartlova | Mineral Wells High School | NT |