| Lane | Team | Squad | Seed |
|---|---|---|---|
| 1 | Columbine High School | A | 54.06 |
| 2 | Ralston Valley High School | A | 50.89 |
| 3 | Chaparral High School | A | 50.40 |
| 4 | Highlands Ranch High School | A | 49.87 |
| 5 | Dakota Ridge High School | A | 50.02 |
| 6 | Chatfield High School | A | 50.46 |
| 7 | Pueblo East High School | A | 53.62 |
| 8 | Mountain Vista High School | A | 54.52 |
| Lane | Team | Squad | Seed |
|---|---|---|---|
| 3 | Conifer High School | A | 59.89 |
| 4 | Evergreen High School | A | 55.32 |
| 5 | Wheat Ridge High School | A | 55.78 |
| 6 | Strive Prep SMART Academy | A | 1:05.83 |