| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Fort Valley State |
A | Cooks II | Jefferson | Jenkins | Williams | DQ | SIAC Championships |
| 2 | Talladega |
A | Emmanuel | Tankard | Louis | Chambers | 40.34 | NAIA Outdoor Track & Field Championships |
| 3 | Morehouse |
A | Alphonse | Smith | Russell II | Simmons jr. | 41.31 | SIAC Championships |
| 4 | Columbus St. |
A | Dillon | Handy | Walls | Tufeiru | 41.31 | Running Eagles Open |
| 5 | Clayton State |
A | Wyman | Hand | Oppong | Peters | 41.86 | Peach Belt Conference Track & Field Championship |
| 6 | Columbus St. |
B | Brown Jr. | Dodd | Parker | Hall | DNS | Running Eagles Open |
| 7 | Morehouse |
B | Alphonse | Banks | Korta | Facey | 41.31 | TFRRS 04/25/24 - 43.45 |
| 8 | Fort Valley State |
B | Adenuga | Givens | Tate Jr. | Rudolph | 40.77 | SIAC Championships |