| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | East Hall HS | A | Bonilla | Hannah | Jackson | Pintado | 1:46.72 | PTR Thursday Night Smackdown |
| 4 | Oconee County High School | A | Elsner | Qualmann | Barnes | Hale | 1:30.52 | George's Lowcountry Table Invite |
| 5 | Jefferson High School | A | Perrin | Glover | Adeyanju | Dowd | 1:28.15 | Jefferson Invitational |
| 6 | Monroe Area High School | A | Vinson | Parks | Kelly | Battle | 1:29.44 | Beach Run Invitational |
| 7 | West Hall High School | A | Aliou | Harrison | Phelps | Wagner | 1:42.34 | Commerce Home Meet #2 |