| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | East Hall HS | A | Williams | Jones | Buffington | Roland | 45.32 | PTR Thursday Night Smackdown |
| 4 | Oconee County High School | A | Osborne | Jackson | Qualmann | Elsner | 42.91 | George's Lowcountry Table Invite |
| 5 | Monroe Area High School | A | Vinson | Denmark | Battle | Kelly | 42.22 | Walton County Track & Field Cham |
| 6 | Jefferson High School | A | Barnes | Little | Perrin | Dowd | 42.75 | Pachuta Insurance Track & Field |
| 7 | West Hall High School | A | Aliou | Phelps | Garcia | Harrison | 44.46 | Hall County Track and Field Cham |